Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZNF317 in samples. An antibody specific for ZNF317 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZNF317 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF317 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF317 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Evaluation of full-length cDNA obtained by RACE showed that it encoded a protein composed of 13 Krpel-like zinc fingers and a KRAB domain. RT-PCR analysis revealed four alternatively splicing products.
The ZNF317 gene has seven exons and is located in human chromosome 19p13. Northern analysis revealed that ZNF317-1 and ZNF317-2 transcripts were ubiquitously expressed, whereas ZNF317-3 and ZNF317-4 transcripts were detected only in lymphocytes, spleen, and lung. Competitive RT-PCR analysis showed that the expression of ZNF317 mRNA significantly decreased during erythroid maturation. In lymphocytes, ZNF317 expression was reduced in response to mitogenic stimulation. ZNF317 may play an important role in erythroid maturation and lymphoid proliferation